Analysis Note

ControlMouse brain tissue lysate

Application

Research CategorySignaling

Research Sub CategoryLipid Metabolism & Weight Regulation

Anti-Fat Free Antibody is a highly specific rabbit polyclonal antibody, that targets Fat free & has been tested in western blotting.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Protein fat-free homolog (Fat Free), also known as Vacuolar protein sorting-associated protein 51 homolog and Another new gene 2 protein (ANG2). Fat Free is a component of the Golgi-associated retrograde protein (GARP) complex which mediates the tethering and fusion of endosome-derived transport carriers to the trans-Golgi network (TGN). The GARP complex maintains protein retrieval from endosomes to the TGN, acid hydrolase sorting, lysosome function, endosomal cholesterol traffic and autophagy. Fat Free participates in acid hydrolase receptor transport, likely by promoting tethering and fusion of endosome-derived carriers to the TGN.

Immunogen

KLH-conjugated linear peptide corresponding to human Fat Free.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Antigen Affinity Purified

Purified rabbit polyclonal in buffer containing PBS with up to 0.1% sodium azide.

Quality

Evaluated by Western Blotting in mouse brain tissue lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected Fat Free in 15 µg of mouse brain tissue lysate.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~80 kDa observed. Uncharacterized bands may appear in some lysate(s). Uniprot describes 2 isoforms at ~73 kDa and ~86 kDa